Fibronectin anticorps

N° du produit ABIN6252186

Cet anticorps Souris Monoclonal détecte spécifiquement Fibronectin dans IHC, IF et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour Fibronectin anticorps (ABIN6252186)

Antigène: Fibronectin

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Fibronectin est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: HFN7-1

Détail du produit anti-Fibronectin anticorps

Purification: Protein G affinity chromatography

Immunogène: Purified human Fibronectin was used as the immunogen for this antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow Cytometry: 0.5-1 μg/million cells

IF: 0.5-1 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Titration of the antibody may be required for optimal performance.

1. FFPE staining requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur Fibronectin

Antigène: Fibronectin

Sujet: The Fibronectin dimer consists of two disulfide linked monomers of approximately 220 kD, and is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. It accumulates at the site of injury and, along with fibrin, forms a blood clot to stop bleeding and protect damaged tissue.

The HFN7.1 Fibronectin antibody is directed against the peptide core and reacts with both the plasma and cellular forms. The antibody blocks the fibronectin-mediated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface.

ID gène: 2335

UniProt: P02751